Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 May 4;93(5):1747–1757. doi: 10.1529/biophysj.106.097907

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, Biophysical Society

PMC Copyright notice

Effective PSF measurement of (A) conventional TIRF, (B) 1D SW-TIRF, and (C) 1D SW-TIRF with linear deconvolution with a 0.04-μm fluorescent bead. The evanescent standing excitation is in the vertical direction. The corresponding vertical intensity profile is (a), (b), and (c). These vertical profiles show a narrower PSF FWHM for SW-TIRF in comparison with that of conventional TIRF. (Experimental condition is the same as that of numerical simulation: NA = 1.45, n = 1.52, and ± 0.5° except the contrast was typically measured ∼0.9.)

Inline graphic — Effective PSF measurement of (A) conventional TIRF, (B) 1D SW-TIRF, and (C) 1D SW-TIRF with linear deconvolution with a 0.04-μm fluorescent bead. The evanescent standing excitation is in the vertical direction. The corresponding vertical intensity profile is (a), (b), and (c). These vertical profiles show a narrower PSF FWHM for SW-TIRF in comparison with that of conventional TIRF. (Experimental condition is the same as that of numerical simulation: NA = 1.45, n = 1.52, and ± 0.5° except the contrast was typically measured ∼0.9.)