Skip to main content
. 2007 May 4;93(5):1758–1767. doi: 10.1529/biophysj.107.108209

FIGURE 5.

FIGURE 5

AFM images of pUC18 molecules irradiated with different doses of UVB radiation and incubated with T4 Endonuclease V: (A) 1.4 kJ/m2-irradiated pUC18, (B) 229 J/m2-irradiated pUC18, (E) 29 J/m2-irradiated pUC18, and (F) intact pUC18 (control experiment). All scan sizes are 1 × 1 μm2. (C, D, G, H) Histograms of the occurrence of various configurations of pUC18 plasmids determined from AFM images such as these shown in (A, B, E, F). Color code and error bar are the same as in Fig. 2. Each histogram is based on 19–36 AFM images. (I) Percentages of different configurations of irradiated pUC18 as a function of UVB dose. (J) The number of CPDs per million basepairs as a function of UVB dose, supposing the distribution of CPDs follows Poisson.