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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2007 Apr 6;1768(6):1311–1324. doi: 10.1016/j.bbamem.2007.03.026

Figure 2.

Figure 2

Modulation of cellular cholesterol by MβCD:MβCD-cholesterol mixtures in Bovine Aortic Endothelial cells (A) and in Chinese Hamster Ovary Cells (B). Modified from Romanenko et al. (2002) [74] and Byfield et al., [135]. MβCD saturated with cholesterol using the standard protocol. Briefly, small volume of a cholesterol stock solution in chloroform:methanol (1:1, vol:vol) was added to a glass tube and the solvent was evaporated. Then, 2.5 mM or 5 mM MβCD solution in DMEM medium without serum was added to the dried cholesterol. The tube was vortexed, sonicated, and incubated overnight in a shaking bath at 37°C. The mixture of MβCD and MβCD -cholesterol was prepared just by mixing the two solutions at different molar ratios.