Figure 4.

Generation of the KZ30.6 stimulating ligand is TAP-dependent. Various numbers of indicated cell lines or normal B6 spleen cells were used as APCs to stimulate KZ30.6 T cells in absence or presence of 10 nM AFV8 peptide. RMA is a tumor cell line from B6 mice, and RMA/S is its TAP⁻ derivative. KZ30.6 response was measured by the lacZ assay as described in Fig. 1.