Figure 2.

RNA blot analysis of huIL-12Rβ2 expression. (Upper) A blot containing 5 μg of poly(A)⁺ RNA from uninduced peripheral blood mononuclear cells (lane 1) or PHA-activated (3 days) peripheral blood mononuclear cells (lane 2) was probed with an IL-12Rβ2 cDNA insert. Two major RNAs at 4.5 kb and 6 kb are apparent in lane 2. Exposure time was 16 hr using an intensifying screen at −80°C. (Lower) As a loading control, the blot was rehybridized with a probe for a cell-cycle independent transcript, pHE7 (37). Exposure time was 20 min using an intensifying screen at −80°C.