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. Author manuscript; available in PMC: 2008 May 18.
Published in final edited form as: Cell. 2007 May 18;129(4):723–733. doi: 10.1016/j.cell.2007.02.050

Figure 4. In HaCaT cells, decreasing the FABP5/CRABP-II ratio diverts RA away from PPARβ/δ.

Figure 4

A. Expression of CRABP-II and FABP5 in mammary tumors that arise in MMTVneu mice and in adjacent normal mammary tissue estimated by immunoblots. B. Expression levels of CRABP-II and FABP5 in HaCaT, NaF, MCF-7, and F9 cells were estimated by immunoblots. C. HaCaT cells were transfected with an expression vector for CRABP-II or with a vector harboring FABP5 siRNA. Effectiveness of transfection in modulating the proteins’ expression levels was monitored by immunoblotting. D. Parental HaCaT cells and HaCaT cells over-expressing CRABP-II or under-expressing FABP5 were treated with denoted ligands (1 μM, 4 hr) and expression of PDK-1 mRNA was measured by Q- PCR. Data are mean ± SEM, n=3.