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. Author manuscript; available in PMC: 2008 May 18.
Published in final edited form as: Cell. 2007 May 18;129(4):723–733. doi: 10.1016/j.cell.2007.02.050

Figure 5. In HaCaT and NaF cells decreasing the FABP5/CRABP-II ratio converts RA from an anti-apoptotic to a pro-apoptotic agent.

Figure 5

A. HaCaT cells were treated with denoted ligands (2 μM, 2 hr) prior to addition of TNFα (20 ng/ml, 14 hr.). Cells were lysed and the level of cleaved PARP assessed by immunoblots. A representative experiment is shown. B. Quantitation of data described in panel a. Band intensities were normalized to the loading control GAPDH. Data are mean ± SEM, n=3. C. and D. Parental HaCaT (C.) or NaF (D.) cells and corresponding cells over-expressing CRABP-II or under-expressing FABP5 were treated with denoted ligands (1 μM, 16 hr.), or pretreated with ligands for 2 hr. prior to addition of TNFα (20 ng/ml, 14 hr., solid bars). Apoptosis was evaluated using the APOPercentage Apoptosis Assay kit. Data are mean ± SEM, n=3.