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She4p interacts with Myo5p in a temperature-dependent manner. Myo5p-TAP from YKT323 lysate was adsorbed to IgG Sepharose. GST-She4p was purified from bacterial lysate. IgG Sepharose with or without Myo5p-TAP was incubated with GST-She4p at 4 or 30°C. The IgG Sepharose was collected, and bound proteins were analyzed by SDS-PAGE and SYPRO orange staining. The result shown is a representative of four experiments.
