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. Author manuscript; available in PMC: 2007 Aug 14.
Published in final edited form as: J Biol Chem. 2002 Sep 5;277(45):43377–43388. doi: 10.1074/jbc.M206373200

Fig. 1. The effect of cisplatin on basal oxygen consumption (QO2) and oligomycin-sensitive QO2 (A) and uncoupled QO2 (B).

Fig. 1

RPTC were treated with 50 μm cisplatin, and samples were taken at 1, 2, 4, 8, 12, and 24 h of cisplatin exposure for measurements of QO2. Control RPTC were treated with vehicle (Me2SO) alone. Basal QO2 in controls did not change over the course of 24 h. Basal QO2 (●) represents the total amount of oxygen consumed by RPTC and was measured as described under “Experimental Procedures.” Oligomycin-sensitive QO2 (○) was measured in the presence of oligomycin (0.6 μg/ml) and calculated as a difference between basal and oligomycin-insensitive QO2. Uncoupled QO2 (■) was measured after addition of FCCP (2 μm). Results are the average ± S.E. of six experiments (RPTC isolations).