Fig. 11. The effect of inhibition of PKC-α and ERK1/2 activation on transient (2 h) and sustained (24 h) changes in ouabain-sensitive oxygen consumption (A) and Na⁺/K⁺-ATPase activity (B) induced by cisplatin (50 μm) in RPTC.

The monolayers were treated and ouabain-sensitive oxygen consumption and Na⁺/K⁺-ATPase activity analyzed as described under “Experimental Procedures.” White columns, controls; black columns, 50 μm cisplatin; light gray columns, 50 μm PD98059 + 50 μm cisplatin; dark gray columns, 10 nm Go6976 + 50 μm cisplatin. Results are the average ± S.E. of three independent experiments (RPTC isolations).