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. Author manuscript; available in PMC: 2007 Aug 14.
Published in final edited form as: J Biol Chem. 2002 Sep 5;277(45):43377–43388. doi: 10.1074/jbc.M206373200

Fig. 12. The effect of cisplatin on caspase-3.

Fig. 12

Caspase-3 activity was quantified by fluorometric detection of free AFC after cleavage from 50 μm DEVD-AFC, and the amount of product cleaved was determined from the AFC standard curve. A, the effect of inhibitors of ERK1/2 activation (50 μm PD98059 and 3 μm UO126) on cisplatin (50 μm)-induced caspase-3 activation. B, the effect of inhibition of PKC-α activation (10 nm Go6976) or treatment with caspase inhibitor (50 μm Z-VAD) on cisplatin (50 μm)-induced caspase-3 activation. Results are the average ± S.E. of four to nine independent experiments (RPTC isolations).