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. Author manuscript; available in PMC: 2007 Aug 14.
Published in final edited form as: J Biol Chem. 2002 Sep 5;277(45):43377–43388. doi: 10.1074/jbc.M206373200

Fig. 3. Mitochondrial hyperpolarization induced by cisplatin exposure in RPTC.

Fig. 3

RPTC were treated with 50 μm cisplatin and loaded with 10 μm JC-1 for 30 min at 37 °C, washed twice with ice-cold PBS, and overlaid with ice-cold PBS. The live RPTC monolayers were examined under Zeiss fluorescent microscope (Axioskop) using water-immersion objective. Original magnification, ×400. A, controls. B, RPTC treated with 50 μm cisplatin for 18 h. C, RPTC treated with 50 μm cisplatin for 24 h. Inset, a single cell (original magnification, ×800). D, mitochondrial depolarization in RPTC induced by FCCP (2 μm). These images are representative of three independent experiments (cell isolations).