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. 2003 Jun;14(6):2327–2341. doi: 10.1091/mbc.02-06-0093

Figure 5.

Figure 5.

A functional role for the ECE-1 3′ UTR. A cytomegalovirus-enhancer-driven luciferase (Luc)/GFP-3 UTR reporter construct containing the proximal 675 base pairs of ECE-1 UTR was introduced and transiently or stably transfected as in MATERIALS AND METHODS. (A) Luciferase activity of transiently transfected stellate cells (transfection for 24 h) is shown. Luciferase activity was normalized to total cellular protein (n = 6). (B) GFP degradation of stably transfected NIH 3T3 cells for up to 12 d is shown. The brightness of fluorescent images was captured at the indicated time points. Images in the first column show control cells without transfection; images in the second column show GFP plasmid transfection; images in the third column show GFP-3′ UTR plasmid transfection; and images in the fourth column show GFP-3′ UTR plasmid transfection in the presence of TGF-β1 (10 ng/ml). The data are representative of two separate experiments.