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. 2003 Jun;14(6):2357–2371. doi: 10.1091/mbc.E02-10-0693

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Figure 3. — Gmh1p is an integral membrane protein. (A) Membrane association of HA-tagged Gmh1p expressed in yeast. Gently lysed RCY006 cells were treated as indicated and then submitted to 100,000 × g centrifugation. Pellet (P) and supernatant (S) fractions were then analyzed by immunoblotting with anti-HA antibodies. (B) Topology of Gmh1p. Gently lysed extracts were prepared from RCY006, YPH499/pAP100 or YPH499/pAP101 strains. Membrane pellets containing the proteins tagged at either Nor C terminus, as indicated, were digested (+) or not (-) with proteinase K and then analyzed by immunoblotting with anti-HA antibodies. Asterisk indicates the shorter polypeptide corresponding to a N-terminus truncated form of Gmh1p.