Phenotypes of hfr1laf1 double mutants under FR light. Seedlings were grown on media without sucrose for 4 d under FR light intensities as indicated. Data are presented as average hypocotyl length ± standard deviations (SD; n = 20). (A) RT–PCR analyses showing reduction of LAF1 transcript in LAF1RNAi/hfr1-201 (panel a) and reduction of HFR1 transcript in HFR1RNAi/laf1 (panel b). (B) Hypocotyl length of wild-type (Col; Columbia, Ler; Lansberg erecta), phyA mutants (phyA-211 and phyA-201), hfr1-201, laf1, LAF1RNAi/hfr1-201, and HFR1RNAi/laf1 seedlings after irradiation with FR (1.5 μmol m−2 sec−1). (Panel a) Measurement of hypocotyl lengths. (Panel b) Comparison of seedling morphology. (C) Responses of hfr1laf1 double mutants under different fluence rates (1, 3, 6, and 10 μmol m−2 sec−1) of FR. (Panel a) Measurement of hypocotyl lengths. (Panel b) Comparison of seedling morphology.