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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: J Neuroimmunol. 2007 May 1;186(1-2):141–149. doi: 10.1016/j.jneuroim.2007.03.021

Fig. 5.

Fig. 5

Modulation of (A) fentanyl- and (B) β-FNA-mediated inhibition of TNFα-stimulated CXCL10 protein expression in A172 cells by naltrexone. Astroglial cells were exposed for 24 h to human recombinant TNFα (5 ng/ml) in the presence or absence of fentanyl (33, 100 and 150 μM), β-funaltrexamine (β-FNA; 11 and 33 μM) and naltrexone (100 μM). A standard dual-antibody solid phase immunoassay (ELISA) was used for quantitation of secreted CXCL10 in cell culture supernatants. Data points represent the mean + S.E.M. of triplicate measures from 2 independent experiments (panel A) and quadruplicate measures from 3 independent experiments (panel B). Data points with any common letters above them are not statistically different from each other as determined by one-way analysis of variance (ANOVA) with Neuman-Kuels’ multiple comparisons.