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Immunoblot analysis of HeLa cell lysates following isopycnic centrifugation in a continuous linear sucrose gradient. A 75-μg sample of protein from each fraction was separated by SDS/10% PAGE, transferred to nitrocellulose, and immunoblotted with Menkes antibody or antibodies to TAP-1, γ-adaptin (AP-1), or the cation-independent mannose-6-phosphate receptor (MPR).
