Table 2.
Comparison of spectroscopic properties of the diiron center in MiaE2H, methane monooxygenase, myo-inositol oxygenase (MIOX-MI), R2 subunit of aerobic ribonucleotide reductase, stearoyl carrier Δ9-desaturase protein, and uteroferrin
| Diiron center | g values | δ (ΔEQ), mm/s FeIII | δ (ΔEQ), mm/s FeII/FeIII | Ref. |
|---|---|---|---|---|
| Mixed-valent form [FeII–FeIII] | ||||
| MiaE2H | 1.91, 1.78, 1.68 | This work | ||
| MIOX-MI | 1.95, 1.81, 1.81 | 0.49 (−1.11) | 1.12 (+2.68) | 25 |
| Ufr | 0.54 (−1.85) | 1.24 (+2.68) | 20 | |
| MMOH | 21 | |||
| Oxidized forms [FeIII–FeIII] | ||||
| MiaE2H (minor) | 0.52 (1.49) | 0.48 (2.16) | This work | |
| RNR | 0.55 (−1.62) | 0.45 (−2.44) | 52 | |
| Ufo | 0.55 (1.69) | 0.48 (2.17) | 20 | |
| MiaE (major) | 0.49 (0.51) | |||
| Δ9D (minor) | 0.49 (0.72) | 23 | ||
| MMOH | 0.50 (0.87) | 0.51 (1.16) | 21 | |