Table 1.

BrdU incorporation and FACSCAN analysis of human diploid RPE1 fibroblasts in the absence or presence of Myc-C-ter AKAP450 expression (low level)

	BrdU incorporation (%)		DNA content (%)
	BrdU-	BrdU+	2N	2N < X < 4N	4N
Nonexpressing RPE1 cells	55	45	58	30	12
Low-expressing Myc-C-ter AKAP450 RPE1 cells	71	28	69	20	11

Twenty-four hours after transfection with the Myc-C-ter AKAP450, RPE1 cells were either incubated with BrdU, fixed, and double immunostained with anti-Myc and anti-BrdU antibodies or analyzed by FACSCAN. Only low expressing RPE1 cells were scored for BrdU staining (>500 cells). DNA contents of nontransfected and Myc-C-ter AKAP450 expressing RPE1 cells are the mean of two separate experiments