Table 1.
Tandem MS identification of Anopheles gambiae midgut microvilli glycoproteins isolated by jacalin-affinity chromatography
| NCBI no. | Ensembl | Annotation | Mascot score | Predicted Mr, kDa | Additional features |
|---|---|---|---|---|---|
| gi_31228112 | ENSANGP00000003366 | Alanyl aminopeptidase N (AgAPN1) | 188 | 113.5 | Chromosome 2L, GPI anchor, Zn-metallopeptidase domain |
| gi_31241511 | ENSANGP00000007943 | Alanyl aminopeptidase N (AgAPN2) | 97 | 104.7 | Chromosome 2R, Zn-metallopeptidase domain, no TMD or GPI anchor |
| gi_31210729 | ENSANGP00000001203 | Alanyl aminopeptidase N (AgAPN3) | 59 | 84.9 | Chromosome 2L, Zn-metallopeptidase domain, no TMD or GPI anchor |
| gi_31230994 | ENSANGP00000024978 | O-glycosyl hydrolase homolog | 52 | 61.5 | Chromosome 2R, glycoside hydrolase family 31, no TMD or GPI anchor, similar to α-galactosidases |
| gi_31210671 | ENSANGP00000013165 | Unknown function | 49 | 19.7 | Chromosome 2L, N-terminal TMD |
NCBI, National Center for Biotechnology Information; TMD, transmembrane domain. Mascot score, probability-based molecular weight search (MOWSE) score. Score, −10 × log 10(P), where P is the probability that the observed match of a protein with the nonredundant database is a random event. The protein scores were ranked, and individual ion scores >51 indicated significant identity (P < 0.05).