Table 1. Backbone amide chemical shift changes on addition of ligands to CBM4-2.
Xylopentaose | Cellohexaose | |||
---|---|---|---|---|
Residue | H | N | H | N |
Gln111*† | 0.29 | 1.22 | 0.21 | 3.17 |
Arg115* | 0.09 | 1.36 | 0.15 | 0.68 |
Arg142* | 0.25 | 0.46 | 0.25 | 0.94 |
Phe110*† | 0.06 | 0.80 | 0.06 | 0.61 |
Glu112* | 0.10 | 0.48 | 0.38 | 0.07 |
Glu118* | 0.08 | 0.38 | NS‡ | NS |
Trp69*† | 0.03 | 0.44 | 0.19 | 0.63 |
Glu72*† | 0.08 | 0.29 | 0.04 | 1.39 |
His146* | NS | NS | 0.07 | 0.27 |
Asp70* | 0.11 | 0.26 | NS | NS |
* Residues targeted by the library design. These residues are listed in order of largest weighted shift change with xylopentaose.
† Residues modified in the X-2 module.
‡ NS, no shift or a value below the cutoff set for a significant shift change.