Table 1. Backbone amide chemical shift changes on addition of ligands to CBM4-2.

	Xylopentaose		Cellohexaose
Residue	H	N	H	N
Gln111*†	0.29	1.22	0.21	3.17
Arg115*	0.09	1.36	0.15	0.68
Arg142*	0.25	0.46	0.25	0.94
Phe110*†	0.06	0.80	0.06	0.61
Glu112*	0.10	0.48	0.38	0.07
Glu118*	0.08	0.38	NS‡	NS
Trp69*†	0.03	0.44	0.19	0.63
Glu72*†	0.08	0.29	0.04	1.39
His146*	NS	NS	0.07	0.27
Asp70*	0.11	0.26	NS	NS

* Residues targeted by the library design. These residues are listed in order of largest weighted shift change with xylopentaose.

† Residues modified in the X-2 module.

‡ NS, no shift or a value below the cutoff set for a significant shift change.