Table 2. Affinities and thermodynamic properties of CBM–carbohydrate interactions as determined by ITC.

Ligand	Protein	KA (M−1)	ΔH (kcal/mol)	TΔS (kcal/mol)	n*
Xylopentaose	CBM4-2	5.13×106	−19.08±0.06	−9.92	0.95±0.002
	X2	4.80×104	−12.65±0.89	−6.26	1.08±0.050
Birchwood xylan	CBM4-2	1.71×105	−15.21±0.29	−8.07	1.06±0.015
	X2	8.39×104	−14.94±1.19	−8.22	1.09±0.066
Oat-spelt xylan	CBM4-2	6.67×105	−17.18±0.39	−9.23	1.07±0.002
	X2	1.15×105	−10.53±1.01	−3.62	0.91±0.060
Wheat arabinoxylan	CBM4-2	1.92×105	−15.69±0.40	−8.46	1.00±0.019
	X2	2.19×104	−18.95±0.71	−13.02	1.01±0.025
β-Glucan	CBM4-2	5.63×104	−12.69±0.40	−6.20	1.03±0.025
	X2	Weak†
Glucomannan	CBM4-2	1.65×103	−16.75±32.4	−12.34	1.02±1.700
	X2	NB‡
Galactomannan	CBM4-2	NB
	X2	NB
Lichenan	CBM4-2	Weak
	X2	NB
Laminarin	CBM4-2	7.82×103	−27.14±9.04	−21.82	0.995±0.260
	X2	NB
Xyloglucan	CBM4-2	Weak
	X-2	NB

* n is the number of binding sites on the protein.

† Extremely weak binding was detected but too low to quantify.

‡ NB, no binding.