Figure 4. DAG induces translocation of the C1 domains of RasGRP1, RasGRP3 and RasGRP4α to membranes.

NIH 3T3 cells expressing the indicated GFP-tagged C1 domains were cultured in serum-free medium for 3.5 h, then treated for 15 min with DMSO (white bars) or 300 μM dioctanoylglycerol (indicated as DAG in the Figure, grey bars). Cells were fractionated and the GFP–C1 proteins in the membrane and non-membrane fractions were quantified as described in the Materials and methods section. Results are means±S.E.M. for two independent experiments.