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. 2007 Aug 13;406(Pt 2):223–236. doi: 10.1042/BJ20070294

Figure 6. Lack of binding of RasGRP C1 domains to alternative lipid ligands.

Figure 6

(A) GST fusions of the indicated RasGRP C1 domains were assayed for binding to PC SLVs (white bars); or PC SLVs containing 10 mol% PS (grey bars), 10 mol% PS and 5 mol% DAG (black bars), or 10 mol% PS and 5 mol% PA (hatched bars). The percentage bound values were not corrected for sedimentation in the absence of lipid. Data are means±range for two independent experiments. RG, RasGRP. (B) GST fusions of the C1 domains of RasGRP1 (white bars), RasGRP2 (black bars), and RasGRP4β (hatched bars) were mixed with PC SLVs containing 5 mol% PS, and 5 mol% of oleic acid (OA), arachidonic acid (AA), ceramide (Cer.), sphingosine 1-phosphate (Sph-1P), LPA or 1 mol% DAG. Data are means±range for two independent experiments.