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. 2007 Aug 13;406(Pt 2):223–236. doi: 10.1042/BJ20070294

Figure 7. RasGRP C1 domains bind to vesicles enriched in anionic phospholipids.

Figure 7

(A) Dependence of C1 domain binding on the mol% of PS. GST fusions of the indicated C1 domains were mixed with SLVs composed of PC and the indicated amounts of PS. Data are means±range for two independent experiments. The curves were generated by the sigmoidal dose-dependence variable slope option of GraphPad Prism, with the exception of the curve for the RasGRP1 C1 domain, which was drawn by hand to give a sigmoidal curve fitting the known percentage bound value of 20% at 5 mol% PS (Figure 5A). (B) C1 domain binding to anionic phospholipids is not headgroup-specific. GST fusions of the indicated C1 domains were assayed for binding to PC SLVs (open bars), 70 mol% PC and 30 mol% PS (black bars), 30 mol% PG (hatched bars), or 30 mol% PA (cross-hatched bars). Data are means± range for two independent experiments. RG, RasGRP.