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. 2007 Aug 13;406(Pt 2):223–236. doi: 10.1042/BJ20070294

Figure 9. The C1 domains of RasGRP1, RasGRP2, RasGRP3 and RasGRP4α are functional within RasGRPs, whereas the C1 domain of RasGRP4β is not.

Figure 9

(A) Structure of RasGRP1 compared with the deleted form of RasGRP1 used to test functionality of attached C1 domains. The GEF (guanine-nucleotide-exchange factor) domains, EF (EF hand domains) and C1 domains are shown, along with the N-terminal GFP tag. (B) NIH 3T3 cells expressing the indicated RasGRP1Δ+C1 fusion proteins or expressing GFP alone as a control, were assessed for oncogenic transformation (low-magnification pictures of cell cultures, left-hand panel, with high refractility and loss of contact inhibition indicating transformation), and for localization of the GFP-tagged proteins (higher-magnification fluorescence microscopy of typical individual cells, right-hand panel). The efficiency of transformation of each construct was determined as described in the Materials and methods section. (C) Structures of GFP-tagged RasGRP4α and β. The bar represents the five-amino-acid insertion in the C1 domain. (D) NIH 3T3 cells were transduced with retroviral vectors expressing the GFP-tagged RasGRP4 constructs and imaged by fluorescence microscopy.