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. 2007 Aug 13;406(Pt 2):317–323. doi: 10.1042/BJ20070286

Figure 3. Knockdown of HIF-1α suppresses cell growth of a HTLV-1-infected T-cell line.

Figure 3

(A) HUT-102 cells were transfected with siRNA for HIF-1α or with a non-target siRNA. The effect of siRNA on cell growth was examined by counting the viable cell number in triplicate using the Trypan Blue dye-exclusion method. (B) RT-PCR analysis showing repression of HIF-1α and VEGF mRNA (one of the target genes of HIF-1α) in HUT-102 cells transfected with HIF-1α siRNA (+) compared with that in cells transfected with non-targeting siRNA (−) 24 and 48 h after transfection. β-Actin expression was used as the cDNA loading control.