Skip to main content
. 2007 Aug 13;406(Pt 2):333–341. doi: 10.1042/BJ20061857

Figure 5. Mouse CD24–Ig produced in LEC12 cells (MoCD24–Ig-LEC12) bound only P-selectin.

Figure 5

(A) Mouse CD24–Ig expressed in LEC12 cells exists as dimer under non-reducing conditions with an apparent molecular mass of 98 kDa. Its apparent molecular mass under reducing conditions is 49 kDa. (B) ELISA plates were precoated with identical amounts of E-selectin–Ig, L-selectin–Ig, P-selectin–Ig or NCAM–Ig fusion protein. Identical amounts of affinity-purified MoCD24–Ig-LEC12 fusion proteins were then added onto the plates. The bound MoCD24–Ig-LEC12 proteins were then detected with rat anti-mouse CD24 antibody and the binding was detected further with goat anti-rat IgG–HRP. MoCD24–Ig-LEC12 fusion protein reacts only with P-selectin. Results are the means±S.D. for three experiments (n=6 for each treatment).