. 2007 Aug 13;406(Pt 2):247–256. doi: 10.1042/BJ20070422

Table 4. Effect of fluoride/chloride leaving group substitution on the rate of catalysis.

The ratio of catalytic rates for the conjugation reaction catalysed by wild-type and engineered GSTs using GSH and CDNB or FDNB as co-substrates. One-way ANOVA with Dunnett's multiple comparison test was performed with wild-type as control; statistical significance is shown by † for P<0.05, ‡ for P<0.01 and § for P<0.001.

	Leaving group effect
Enzyme	k_cat^FDNB/k_cat^CDNB	(k_cat/K_m)^FDNB/(k_cat/K_m)^CDNB
Wild-type	1.76±0.01	20.59±1.02
H38A	3.11±0.21	14.12±0.84†
M39A	8.29±0.95§	32.45±3.72§
N47A	18.09±0.90§	29.65±1.32‡
Q49A	2.63±0.08	26.29±1.77†
Q49E	1.50±0.08	24.95±1.21
H50A	18.57±0.41§	16.55±1.59
C51A	2.93±0.05	30.31±2.85§
C51D	26.75±0.99§	44.71±4.15§