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. 2007 Aug 13;406(Pt 2):355–363. doi: 10.1042/BJ20070363

Figure 3. Expression and purification of recombinant LALP toxin analysed by SDS/PAGE (12% gel) under reducing conditions and Coomassie Blue staining.

Figure 3

Lane 1, E. coli BL21(DE3)pLysS cells collected by centrifugation and resuspended in Laemmli sample buffer 3.5 h prior to induction with 0.025 mM IPTG. Lane 2, the supernatant of cell lysates induced with 0.025 mM IPTG, obtained by freeze–thawing and sonication in extraction buffer before affinity chromatography using a Ni-NTA–agarose column. Lane 3, purified recombinant protein after elution through a Ni-NTA–agarose column. Molecular-mass standards are shown on the left.