Figure 2. mICA protein and mRNA expression levels in various mouse strains with mutations in genes involved in iron metabolism.

(A–D) Western blots of serum from control and transgenic mice as indicated. (E) Northern blots of 10 μg of total RNA from Trf^hpx/+ or Trf^hpx/hpx mice. The blots were probed using either a 587 bp ³²P-labelled fragment containing the mouse TF ORF (1–587 bp) (left) or a 585 bp ³²P-labelled fragment containing the mouse mICA ORF (1–585 bp) (right). The molecular masses are indicated on the left. After stripping, the blots shown on the bottom were reprobed with the loading control mouse actin.