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. 2007 Jul 26;406(Pt 1):85–95. doi: 10.1042/BJ20070384

Table 2. Oligonucleotide sequence of the 5′-end of the mICA construct.

The 5′-end of the mICA construct is shown (mICA residues are denoted with superscript numbers, the reading frame is indicated by spaces, and the NotI site is underlined).

M R L A V G A L L V C A V L G L
5′-GCGGCCGCACC ATG AGG CTC GCC GTG GGA GCC CTG CTG GTC TGC GCC GTC CTA GGG CTG
3′-CGCCGGCGTGG TAC TCC GAG CGG CAC CCT CGG GAC GAC CAG ACG CGG CAG GAT CCC GAC
C L A V P D K H H H H H H I E G R L20 P21
TGT CTG GCT GTC CCT GAT AAA CAT CAT CAT CAT CAT CAT ATC GAG GGA AGG CTT CCT-3′ (sense)
ACA GAC CGA CAG GGA CTA TTT GTA GTA GTA GTA GTA GTA TAG CTC CCT TCC GAA GGA-5′ (antisense)