Figure 1. Titration of succinate-promoted H₂O₂ generation.

(A) Different rates of H₂O₂ production are elicited by GM and succinate. Succinate (trace a) and GM (trace b) were 1 mM. Mitochondria (0.75 mg/1.6 ml) were incubated in standard incubation medium containing HRP. Amplex Red was added at the time indicated by the arrow. (B) H₂O₂ production was measured in the absence (○) and presence of GM, 1 mM (Δ) or 2 mM (▲) each. Where present, rotenone was 2.5 μM (●) without GM. (C) Net H₂O₂ releases calculated by subtracting the rotenone rates from the rates in the absence of rotenone in (B). Traces in (A) are representative of duplicate traces from at least seven independent experiments. Results in (B) are means±S.D. for at least five independent experiments.