Table 1. O2 consumption rate measured in mitochondria oxidizing different respiratory substrates.
Mitochondria (0.9 mg/1.6 ml) were incubated in standard medium supplemented with Amplex Red and HRP, and respiration rates were measured. GM (1 mM each), succinate (0.5 mM) and rotenone (1 μM) were present or added as indicated. Results are means±S.D. for at least seven independent experiments.
| Substrate | Rate of O2 consumption (n-atoms/min per mg) |
|---|---|
| GM | 10.5±2.5 |
| GM + succinate | 16.8±2.0 |
| Succinate | 28.0±3.0 |
| Succinate + GM | 17.5±1.2 |
| Rotenone/GM/succinate | 27.5±2.0 |