Figure 2. Induction of NOX4 mRNA and ROS release as a function of tet concentration in inducible NOX4 cells.

NOX4 T-REx™ cells (clone 20) were incubated for 24 h with tet (1.25 fg/ml to 10 μg/ml). The ROS release is presented as relative scopoletin fluorescence/h (100 corresponds to cells induced with 1 μg/ml tet, TC). The dose–response curve was fitted with a four-parameter Hill sigmoid curve for the determination of the EC₅₀ (n=4).