Figure 4. NOX4 is an NADPH-dependent oxidase.

T-REx™ NOX4 cells were induced with 1 μg/ml tet (TC) for 24 h, followed by lysis and membrane preparation. Membranes (5 μg/well; no addition of cytosol) were added to an Amplex Red mixture in a final volume of 200 μl. NADPH and NADH were added, and mixtures were incubated for 20 min at 37 °C before ROS production was measured by Amplex Red fluorescence. Results (means±S.E.M.) are shown as end values (n=3–4). **P<0.01.