Figure 9. Intracellular ROS detection with NBT is positive for NOX4-expressing cells.

NBT (1.6 mg/ml) was added to cells and plates were incubated at 37 °C for 45 min. Micrographs show NBT tests from (a) non-induced NOX4 cells and (b) tet (TC)-induced NOX4 cells. (c) Quantification of dissolved formazan by absorbance quantification at 630 nm from non-induced and tet (TC)-induced NOX4 cells (n=3–5). ***P<0.001. OD, absorbance; WT, wild-type.