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. 2007 Jul 26;406(Pt 1):157–165. doi: 10.1042/BJ20070359

Figure 2. U73122 blocking effect on TGN to plasma membrane transport.

Figure 2

(A) HeLa cells were transfected with the thermosensitive mutant GFP–VSV-G tsO45. After accumulation of this protein in TGN at 20 °C, the cells were incubated at the permissive temperature for 60 min, and then analysed by immunofluorescence to monitor the localization of VSV-G. All the procedure was performed in the presence of U73122 or U73343, except for non-treated cells (NT). (B) Measurement of the experiment described in (A), depicted as a percentage of GFP–VSV-G-expressing cells where this protein has reached the cell surface. Values are the means (±S.D., vertical bars) for three separate experiments.