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. 1996 Nov 26;93(24):14036–14040. doi: 10.1073/pnas.93.24.14036

Figure 4.

Figure 4

(A) There was an increased amount of Raf-1 protein associated with the membrane in R2 over expressing cells. The recombinant R2-expressing cell lines B3/mR2, N3/mR2, C1/mR2, r-2/mR2, r-3/mR2, and NR4/mR2 (R2) were compared with their respective control lines, B3/SH, N3/SH, C1/SH, r-2/SH, r-3, and NR4 (control). In all cases, cells expressing recombinant R2 exhibited increased membrane-associated Raf-1 protein, and when the two groups of cell lines were compared, they were found to be significantly different by t test analysis (P < 0.001). (B) There was also an increase in the activity of MAPK-2 in R2 overexpressing cells. The recombinant R2-expressing lines B3/mR2, N3/mR2, 10T/mR2, C1/mR2, r-2/mR2, and NR4/mR2 (R2) were compared with their respective control lines infected with LXSH (controls). In all cases tested, cells expressing recombinant R2 showed increased enzyme activity, and the difference between two groups was highly significant (P < 0.001). (C) Increased focus formation with N3/mR2 cells compared with N3/SH cells after transfection with the activated V12 Rac-1 plasmid. The difference was highly significant (P < 0.001). The number of foci shown represents the average ± SE from two independent experiments.