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. 2003 Jun;14(6):2530–2542. doi: 10.1091/mbc.E02-12-0790

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Figure 8. — Indirect immunofluorescence localization of Fus1 to the fertilization tubule of activated mt+ gametes. (A) Anti-Fus1 indirect immunofluorescence of wild-type mt+ gametes. (B and B′) Corresponding micrographs of wild-type mt+ gametes dual-labeled with the Fus1 polyclonal antibody (B, green) and the filamentous actin-specific fluorochrome Alexa 546-phalloidin (B′, red). (C and C′) Corresponding micrographs of fus1-1 mt+ gametes dual-labeled with the Fus1 polyclonal antibody (C) and the actin-specific fluorochrome Alexa 546-phalloidin (C′). The arrowheads point to the position of fertilization tubules.