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. 2007 Jul 19;26(15):3673–3685. doi: 10.1038/sj.emboj.7601795

Figure 2.

Figure 2

Characterisation of M. grisea Δhxk1 mutants. (A) Hexokinase activity was measured and compared in Δtps1, Δhxk1, Δtps1 Δhxk1 and Guy-11 strains. Strains were grown in complete medium (CM) for 48 h followed by growth in glucose minimal medium (GMM) for 16 h. (B) G6P levels in the same strains and the same conditions. Bars represent standard error of three independent analyses. (C) Trehalose levels in the same strains and conditions. Bars represent standard error of three independent analyses. (D) Effect of Δhxk1 mutation on sporulation. Δtps1, Δhxk1, Δtps1 Δhxk1 and Guy-11 strains were grown on GMM plates (n=4) for 10 days, followed by harvesting of the spores for counting. Bars represent standard error. (E) Effect of Δhxk1 mutation on vegetative growth. Δtps1, Δhxk1, Δtps1 Δhxk1 and Guy-11 strains were grown on GMM plates for 10 days. (F) Virulence of Δtps1, Δhxk1, Δtps1 Δhxk1 and Guy-11 strains on rice seedlings. Rice seedlings were inoculated at a concentration of 5 × 104 spores/ml. (G) Hexokinase inhibition by 1 mM T6P. Bars represent standard error of three independent analyses.