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SRC-11051−1240-YFP recruitment to the PRL DNA array in the presence of tamoxifen. Staining of RNA polymerase II was performed on PRL array containing HeLa cells, transfected with ERαwt-CFP and SRC11051−1240-YFP and cultured in medium containing only CTS. Subsequently, cells were treated with 1 μM E2 or 1 μM 4′OH-tamoxifen for 2 h and then fixed and stained for RNA polymerase II. Arrowheads indicate the PRL DNA array that was analyzed for RNA polymerase II staining.
