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The RING-H2 domain is essential for oligomerization of full-length Ste5. (A) Cartoon of Ste5 and its binding partners. LZ, leucinezipper. (B) Ste5 must have a RING-H2 domain to oligomerize efficiently. Coimmunoprecipitation of Myc- and HA-tagged derivatives of Ste5 by using extracts from ste5Δ (EY1775) cells expressing HA3-Ste5Δ474-638 (pYMW 68) with Ste5-Myc9 (pSKM19), Ste5ΔRING-Myc9 (pLS46), or Ste5Δ474-638-Myc9 (pYMW13).
