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Ste5Δ474-487 and Ste5L482/485A are more efficiently exported than wild-type Ste5. (A) Mutation of the Msn5 exportin relocates Ste5Δ474-487 and Ste5L482/485A to the nucleus. Indirect immunofluoresence of vegetatively dividing wild-type (EY699) and msn5Δ (ELY357) cells expressing Ste5-Myc9 (pSKM19), Ste5Δ474-487-Myc9 (pYMW4), and Ste5L482/485A-Myc9 (pYMW37). (B) Quantitation of nuclear accumulation in MSN5 and msn5Δ cells.
