Table 1.
Yeast strains and plasmids used in this study
Strains/plasmids | Genotype/description | Source | ||
---|---|---|---|---|
EY699 | ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 can1-100 | R. Rothstein | ||
EY957 | bar1Δ | Elion et al. (1993) | ||
EY1775 | bar1Δ ste5Δ::TRP1 | Choi et al. (1994) | ||
EYL357 | msn5::HIS3 | Mahanty et al. (1999) | ||
EY2320 | ste11Δ | Lee and Elion (1999) | ||
EYL1808 | ste4Δ::LEU2 ste5Δ::TRP1 | Feng et al. (1998) | ||
EYL1809 | STE11-4 ste5Δ::TRP1 far1Δhis3Δ200 lys2::FUS1-HIS3 | Feng et al. (1998) | ||
BY4741 | leu2 ura3 his3 met15 | Research Genetics | ||
2468 | ste4Δ::KANR leu2 ura3 his3 met15 | Research Genetics | ||
PSY580 | ura3-52 trp1Δ63 leu2Δ1 | Seedorf and Silver (1997) | ||
PSY1103 | rsl1-4 ura3-52 trp1Δ63 leu2Δ1 | Seedorf and Silver (1997) | ||
LDY375 | nsp1ts::URA3 ade2 leu2 lys1 | Nehrbass et al. (1993) | ||
pYMW4 | ste5Δ474-487-MYC9 | 2μ | URA3 | This study |
pYMW7 | ste5Δ474-487 | CEN | URA3 | This study |
pYMW13 | ste5Δ474-638-MYC9 | 2μ | URA3 | This study |
pYMW27 | TAgNLS-GFP-GFP | 2μ | URA3 | This study |
pYMW37 | ste5L482/485A-MYC9 | 2μ | URA3 | This study |
pYMW39 | ste5L482/485A | CEN | URA3 | This study |
pYMW49 | ste5Δ474-487-GST | 2μ | URA3 | This study |
pYMW50 | STE5-GST | CEN | URA3 | This study |
pYMW57 | ste5Δ474-487-GST | CEN | URA3 | This study |
pYMW64 | ste5L482/485A-GST | 2μ | URA3 | This study |
pYMW66 | HA3-ste5L482/485A | 2μ | LEU2 | This study |
pYMW68 | HA3-ste5Δ474-638 | 2μ | LEU2 | This study |
pYMW74 | HA3-STE5-GST | 2μ | LEU2 | This study |
pYMW77 | STE5-GST | 2μ | URA3 | This study |
pYMW81 | TAgNLS-STE5-GST | CEN | URA3 | This study |
pYMW82 | TAgNLS-STE5-GST | 2μ | URA3 | This study |
pYMW96 | GAL1-HA-STE4 | CEN | LEU2 | This study |
pYMW97 | ste5C180A-GST | 2μ | URA3 | This study |
pYMW98 | ste5C180A-GST | CEN | URA3 | This study |
pYMW99 | ste5ΔNLS(Δ49-66)-GST | 2μ | URA3 | This study |
pYMW100 | ste5ΔNLS(Δ49-69)-GST | CEN | URA3 | This study |
pYMW102 | ste5L482/485A-GST | CEN | URA3 | This study |
pYMW106 | HA3-ste5Δ474-487 | 2μ | LEU2 | This study |
pYMW134 | MYC3-STE5-GST | 2μ | URA3 | This study |
pYMW138 | MYC3-STE5 | 2μ | URA3 | This study |
pSKM19 | STE5-MYC9 | 2μ | URA3 | Mahanty et al. (1999) |
pSKM27 | GAL1-TAgNLS-STE5 | 2μ | URA3 | Mahanty et al. (1999) |
pSKM31 | ste5C180A-MYC9 | 2μ | URA3 | Feng et al. (1998) |
pSKM44 | TAgNLS-STE5 | CEN | URA3 | Mahanty et al. (1999) |
pSKM47 | TAgNLS-STE5 | 2μ | URA3 | Mahanty et al. (1999) |
pSKM87 | HA3-STE5 | 2μ | LEU2 | Mahanty et al. (1999) |
pSKM90 | STE5-MYC9 | 2μ | HIS3 | Mahanty et al. (1999) |
pSKM98 | TAgNLSK128T-STE5-MYC9 | 2μ | URA3 | Mahanty et al. (1999) |
pLS46 | ste5ΔRING-MYC9 | 2μ | HIS3 | Feng et al. (1998) |
pYBS138 | STE5 | CEN | URA3 | Kranz et al. (1994) |
pYEE102 | FUS3-HA5 | CEN | HIS3 | Elion et al. (1993) |
pKC55 | GAL1-STE7-MYC | CEN | HIS3 | Choi et al. (1994) |
pNC245 | GAL1-STE11-MYC | CEN | TRP1 | Choi et al. (1994) |
pJB207 | FUS1-LACZ | 2μ | LEU2 | Elion et al. (1990) |
All yeast strains are MATa. The EY and EYL strains are isogenic derivatives of W303a