Table 2.

Localization of wild-type and mutant derivatives of Ste5

				% Nuclear accumulation		% Rim staining
Plasmids	Strainsa	Coexpressed with	Carbon sourceb	- α F	+ α F	+ α F
1. Ste5-Myc9	ste5Δ		Dex	24±0.9		12±1.9
2. Ste5Δ474-487-Myc9	ste5Δ		Dex	0±0.6		0±0
3. Ste5L482/485-Myc9	ste5Δ		Dex	6±2.4		3±0.6
4. Ste5-Myc9	STE5		Dex	29±4.8		16±2.7
5. Ste5Δ474-487-Myc9	STE5		Dex	10±2.7		0±0.2
6. Ste5L482/485-Myc9	STE5		Dex	14±2.4		9±2.2
7. Ste5-Myc9	ste11Δ		Dex	21±1.6
8. Ste5-Myc9	ste5Δ	Vector	Dex	18±6.4	8±3.1	17±5.0
9. Ste5-Myc9	ste5Δ	ADH2-p-TAgNLS-(GFP)2	Dex	15±5.6	6±1.4	14±4.4
10. Ste5-Myc9	ste5Δ	STE5-p-TAgNLS-STE5	Dex	28±7.9	14±2.8	41±5.0
11. Ste5-Myc9	ste5Δ	ADH2-p-TAgNLS-(GFP)2	Gal	23±1.7	7±1.5	24±1.8
12. Ste5-Myc9	ste5Δ	GAL1-p-TAgNLS-STE5	Gal	52±0.9	34±2.5	41±1.9

^aStrains were EY1775 (ste5Δ), EY957 (STE5) and EY2320 (ste11Δ)

^bCells were grown in 2% of either dextrose (Dex) or galactose (Gal) medium