Figure 4.

c-Myb and Gata3 expression following TCR signalling. (A) qRT–PCR quantitating c-Myb expression relative to hprt. mRNA was harvested from naïve DP thymocytes stimulated for the times shown on the x-axis with nothing (grey lines), or αCD3 (black lines). Data are the mean of three experiments performed in triplicate. Error bars: s.d. (B) qRT–PCR quantitating Gata3 mRNA relative to hprt mRNA from the same experiments. (C) Gata3 expression assessed by Western blot relative to a β-actin control. Cell lysates were prepared from purified DP CD69⁻ thymocytes of the genotypes shown, stimulated in vitro with PMA and ionomycin for the indicated times. (D) c-Myb and Gata3 expression following αCD3 stimulation of MHCI/II^o/o DP thymocytes in the presence or absence of UO126. Data are the mean of three experiments performed in triplicate. Error bars: s.d.