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. 2007 Jul 19;26(15):3629–3640. doi: 10.1038/sj.emboj.7601801

Figure 5.

Figure 5

c-Myb regulates Gata3. (A) Downregulation of Gata3 upon inhibition of Myb. E16C cells were treated with 4-OHT, to induce MERT and hence repress Myb activity, or vehicle control for 24 h. qRT–PCR was performed to quantitate Gata3 (black bars) or Bcl2 (white bars) expression relative to hprt. Data are the mean of five experiments. Error bars: s.d. (B) EMSA using as probe a strong Myb-binding site (MybMBS). Lanes 1 and 2; blank reticulocyte lysate controls. Lanes 3–9; reticulocyte lysate programmed with the Myb DNA-binding domain (MT). Lane 3; no competitor. Lane 4; 10-fold excess unlabelled wt MybMBS. Lanes 5–9: 100-fold excess unlabelled mutant MybMBS (lane 5); wt MBS G1 (lane 6); mutant MBS G1 (lane 7); wt MBS G2 (lane 8), or mutant MBSG2 (lane 9). (C) Chromatin immunoprecipitation. PCR reactions from immunoprecipitated DNA specific for the indicated regions are shown. Data were identical in two separate experiments.