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. Author manuscript; available in PMC: 2008 Jun 8.
Published in final edited form as: Neurosci Lett. 2007 Apr 5;420(1):53–57. doi: 10.1016/j.neulet.2007.04.005

Figure 4.

Figure 4

Western blots of GFP (A) and CaB (B). Reactions containing lysates of non-transfected HeLa cells and cells expressing ataxin-1 (Q2, 30 or 82) were incubated for 30 min at room temperature with rat brain CaB followed by the addition of 0.1 mM protein-protein cross-linker DSS. The reactions were then incubated for additional 15 min. In the presence of DSS ataxin-1 formed insoluble aggregates due to self-association (A). A decrease in the intensity of CaB bands in Q30 and Q82 lanes (in the presence of DSS) indicate that CaB may preferably interact with Q30 and Q82 ataxin-1 (B).