Immature moDC were mock infected or infected at high moi with WT rSV5. At 6 h pi, cells were left untreated (UT samples) or treated with 10 ng/ml IL-1beta. Levels of cell surface CD40 (panel A), CD80 (panel B), and intracellular cleaved caspase-3 (panel C) were determined by flow cytometry. Results are the average of mean fluorescent intensities from three experiments using three individual donors (with standard error bars). CD40 and CD80 results are expressed as a fold change relative to mock infected, mock treated sample set at 1. *, P values for CD40 and CD80 on IL-1beta treated infected cells compared to untreated infected control cells were 0.007 and 0.02, respectively. #, P values for percent active caspase-3 on IL-1beta treated infected cells compared to untreated infected control cells was 0.05.